The Denison Police Department is currently staffed with twelve sworn full time officers and seven civilian employees. Chief Rod Bradley leads the department. Chief Bradley joined the department in 1980 as a patrol officer and served as a night sergeant and Asst. Chief prior to his appointment as Chief of Police in June of 1992. The Police Department has three sergeants, Sgt. Ken Schwenn, Sgt. John Emswiler and Sgt. Alison Dorhout, and eight patrol officers. Sgt. Schwenn and Sgt. Emswiler coordinate the narcotics investigations for the department and both are former D.A.R.E. officers. Sgt. Dorhout is currently the senior D.A.R.E. Instructor for the department.

In addition to providing police protection throughout the city the police department also staffs and manages the County-Wide E-911 Center. The Communications Center is the only public service answering point in Crawford County and is responsible for dispatching eight fire departments and their EMS first responders, the Crawford County Memorial Hospital Ambulance Service, the Crawford County Sheriffs Office, and the Denison, Manilla, Dow City and Charter Oak Police Departments.